![]() ![]() If the recorder has a problem, here are some examples of macro line: OtherMeasuresResults-A/B gives measurements on each objects from images A and B.Curve of the shuffle (cumulative distances).DistanceResults gives measurements on non co-localizing objects.ColocResults gives measurements on co-localizing objects.Number of objects and number of co-localizing objects are given in the log window.Objects from image A and B are listed in a ROI Manager.If images opened are already calibrated, the plugin reads the calibration (otherwise it will ask you to inter the values). Resolution infos Image calibration has to be given for the measurements to be calibrated. Select to do a shuffle of the objects on all the image or inside a maskĬhoose the parameters that will be measured for each objects Measures & AnalysisĬhoose the parameters that will be measured for co-localizing objectsĬhoose the parameters that will be measured for non co-localizing objects When unchecking « All objects touching », the plugin will analyse the non co-localizing objects in addition to co-localizing objects. Filter objects by size is possible Interactions filters Iterative segmentation method : a tutorial for this method is found here Spot segmentation method : a tutorial for this method is found here Segment the image with either threshold or spot segmentation method The image A is the reference image.įilter objects by size and remove objects from image edges is possibleĪpply filter to suppress noise (optional) This plugin does not support RGB and 32-bits images. Note that when the mouse arrow passes on the items of the plugin, an explanation of each parameter is given. Quantifies for each found object the following parameters: Generates results representations such as:Ĭounts the number of 3D objects in two stacks.Colocalization from objects in image A, B, and/or A+B.Object based co-localization and distance analysis 3D Segmentation (iterative thresholding, spots segmentation, …).Thomas Boudier, CENTURI, Université Aix-Marseille, France. Jean-François Gilles, Institute of Biology Paris Seine (France). The plugin can be used with labelled images, but it also integrates tools for the segmentation of the objects. Getting some 3D measurements about each objects.Measuring 3D distances between nearest object, co-localized or not.Calculating co-localization between objects in 3D.If you’d like to help, check out the how to help guide! Distance Analysis (DiAna) The content of this page has not been vetted since shifting away from MediaWiki. ![]()
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